Types Of Tube-Related Blood Collection Errors

When it comes to collecting blood samples for testing, healthcare professionals must follow strict protocols to ensure accurate results. However, errors can occur during the collection process, leading to inaccurate test results and potentially affecting patient care. One common source of errors in blood collection is related to the tubes used to collect the samples. In this article, we will discuss the types of tube-related blood collection errors that healthcare professionals should be aware of.

1. Incorrect Order of Draw

One of the most common tube-related blood collection errors is the incorrect order of draw. The order in which tubes are filled during blood collection is crucial to prevent cross-contamination and ensure accurate test results. The correct order of draw is as follows:

  1. Lavender (EDTA) tubes for hematology tests

  2. Light blue tubes for coagulation tests

  3. Red or tiger top tubes for chemistry tests

  4. Green tubes for plasma tests

Failure to follow the correct order of draw can lead to contamination of the samples with additives from previous tubes, resulting in inaccurate test results.

2. Tube Mislabeling

Another common tube-related blood collection error is mislabeling of tubes. When collecting blood samples, it is essential to label each tube with the patient's information, including their name, date of birth, and medical record number. Mislabeling can lead to confusion and mix-ups in the laboratory, resulting in inaccurate test results being reported for the wrong patient.

Healthcare professionals should double-check that each tube is properly labeled before sending it to the laboratory for testing. Using electronic barcode systems can also help reduce the risk of mislabeling errors.

3. Incorrect Tube Selection

Choosing the wrong tube for a specific test can also lead to blood collection errors. Different tubes contain different additives to facilitate specific tests, and using the wrong tube can result in inaccurate test results. Healthcare professionals should be familiar with the types of tubes used for different tests and ensure they are using the correct tubes during blood collection.

For example, using a green tube instead of a light blue tube for coagulation tests can lead to inaccurate results due to the presence of additives in the wrong tube. Healthcare professionals should consult laboratory guidelines and protocols to ensure they are using the correct tubes for each test.

4. Overfilling or Underfilling Tubes

Incorrectly filling blood collection tubes can also lead to errors in test results. Overfilling or underfilling tubes can affect the ratio of blood to additive in the tube, potentially impacting the accuracy of test results. Tubes should be filled to the specified volume indicated on the tube to ensure proper mixing of the blood with the additive.

Healthcare professionals should be trained on proper blood collection techniques to avoid overfilling or underfilling tubes. Using collection devices with volume indicators can help ensure tubes are filled to the correct volume.

5. Tube Mixing Errors

After collection, blood tubes containing additives must be gently inverted to ensure proper mixing of the blood with the additive. Failure to mix tubes adequately can result in inadequate anticoagulation, leading to clot formation or inaccurate test results. Healthcare professionals should follow manufacturer instructions for tube mixing to ensure proper sample preparation.

Improper mixing can also result in sample hemolysis, which can affect the accuracy of certain tests. Healthcare professionals should be trained on proper tube mixing techniques to prevent these types of errors.

By being aware of the types of tube-related blood collection errors discussed in this article, healthcare professionals can take steps to prevent these errors and ensure accurate test results for their patients. Following proper blood collection protocols, labeling tubes correctly, using the appropriate tubes for each test, and avoiding overfilling or underfilling tubes can help minimize the risk of tube-related errors in blood collection.

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Lauren Davis, BS, CPT

Lauren Davis is a certified phlebotomist with a Bachelor of Science in Public Health from the University of Miami. With 5 years of hands-on experience in both hospital and mobile phlebotomy settings, Lauren has developed a passion for ensuring the safety and comfort of patients during blood draws. She has extensive experience in pediatric, geriatric, and inpatient phlebotomy, and is committed to advancing the practices of blood collection to improve both accuracy and patient satisfaction.

Lauren enjoys writing about the latest phlebotomy techniques, patient communication, and the importance of adhering to best practices in laboratory safety. She is also an advocate for continuing education in the field and frequently conducts workshops to help other phlebotomists stay updated with industry standards.

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Blood Sample Contamination During Collection

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Best Practices In Blood Collection