Understanding Order Of Draw

The order of draw is a critical concept in phlebotomy, the process of drawing blood from patients for medical testing purposes. Proper order of draw ensures accurate and reliable test results, prevents sample contamination, and minimizes the risk of needlestick injuries. In this blog post, we will explore the importance of the order of draw, the correct sequence to follow, and tips for phlebotomists to remember. Let's dive in!

Importance of Order of Draw

The order of draw is essential in phlebotomy because different blood collection tubes contain various additives that can interfere with test results if drawn in the wrong order. For example, some tubes contain anticoagulants to prevent blood clotting, while others contain clot activators to promote coagulation. Drawing blood in the incorrect order can lead to false results and impact patient care decisions.

Additionally, following the correct order of draw helps prevent sample contamination. When blood is drawn, there is a risk of residual additives from previous tubes affecting subsequent samples. By following a standardized sequence, phlebotomists can minimize the risk of cross-contamination and ensure the integrity of each blood sample.

Correct Sequence of Order of Draw

The standard order of draw used in most healthcare settings is as follows:

1. Yellow or SPS tube: This tube contains sodium polyanethol sulfonate and is used for blood culture tests to detect infections.
2. Light blue tube: This tube contains sodium citrate and is used for coagulation tests, such as PT and PTT.
3. Red or Gold tube: This tube may contain clot activator, gel separator, or no additives and is used for various chemistry tests.
4. Green tube: This tube contains heparin and is used for plasma determinations in chemistry and electrolyte studies.
5. Lavender or Purple tube: This tube contains EDTA and is used for hematology tests, such as CBC and blood typing.
6. Gray tube: This tube contains potassium oxalate and sodium fluoride and is used for glucose testing.

Tips for Remembering the Order of Draw

It can be challenging for phlebotomists to remember the correct sequence of tubes, especially when faced with a busy and fast-paced environment. Here are some tips to help you remember the order of draw:

1. Use mnemonic devices: Create a simple phrase or acronym using the first letter of each tube color to help you recall the correct order.
2. Practice regularly: Repetition is key to memorizing the order of draw. Take every opportunity to practice and quiz yourself on the sequence.
3. Refer to a chart: Keep a reference chart or poster in your workspace to consult when needed.

Conclusion

Understanding the order of draw is crucial for phlebotomists to perform their job accurately and efficiently. By following the correct sequence of blood collection tubes, healthcare professionals can ensure reliable test results, prevent sample contamination, and prioritize patient safety. Remembering the order of draw may take time and practice, but it is a valuable skill that will benefit both phlebotomists and their patients.

For more information on the order of draw in phlebotomy, check out the following resources:

1. ASCLS.org - Order of Draw
2. UpToDate - Order of Draw for Multiple Tube Collections
3. LabCE - Order of Draw Study Guide

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